THE POTENTIOMETRIC UREA BIOSENSOR USING CHITOSAN MEMBRANE
| Main Authors: | Mulyasuryani, Ani; Chemistry Department, Brawijaya University Jalan Veteran, Malang, Roosdiana, Anna; Chemistry Department, Brawijaya University Jalan Veteran, Malang, Srihardyastutie, Arie; Chemistry Department, Brawijaya University Jalan Veteran, Malang |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Gadjah Mada
, 2010
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| Online Access: |
http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/view/569 http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/view/569/582 |
Daftar Isi:
- Potentiometric urea biosensor development is based on urea hydrolysis by urease resulted CO2. The biosensor is used chitosan membrane and the H3O+ electrode as a transducer. The research was studied of effecting pH and membrane thickness to the biosensor performance. The best biosensor performance resulted at pH = 7.3 and 0.2 mm of membrane thickness. The biosensor has a Nerntian factor 28.47 mV/decade; the concentration range is 0.1 up to 6.00 ppm; and the limit of detection is 0.073 ppm. The response time of this biosensor is 280 seconds, efficiency 32 samples and accuracy 94% up to 99%. Keywords: biosensor, potentiometry, urea, chitosan membrane