MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION
| Main Authors: | Aprilita, Nurul Hidayat; Chemistry Department, Faculty of Mathematics and Natural Sciences, Gadjah Mada University,, Bakry, Rania; Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,, Huck, Christian W; Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,, Bonn, Guenther K; Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Gadjah Mada
, 2010
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| Online Access: |
http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/view/196 http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/view/196/270 |
Daftar Isi:
- Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography. Keywords: monolithic disk, poly(styrene/divinylbenzene), proteins