thermodynamics equilibrium constants generalization nucleobase spectral deconvolution spectral unmixing equilibrium constant nucleoside biocuration biomolecules breakeven calculation cascade reactions community formation coupled enzyme reactions coupled reactions curation database enzyme cascade reactions enzyme-catalyzed reactions error propagation literature review measurement nucleosides open database reaction monitoring uncertainty uv vis spectroscopy benchmark chemistry comparison degradation fluoro-modified nucleoside derivatives fluoro-nucleosides isosbestic point matrix factorization normalization nucleoside phosphorylase product isolation FAIR data Gibbs energy of reaction RDM assay biochemical pathways chemical reaction network data management enzyme reaction experimental work graph