1 Institut Pertanian Bogor

1 HAYATI Journal of Biosciences 1 Jurnal Matematika dan Aplikasinya 1 Jurnal Mikrobiologi Indonesia

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1 340 bps with an open reading frame of 1 1 85.9 1 962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa 1 GgVLG can be used as a molecular marker for giant gourami germ cells 1 Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study 1 a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2 more ... 1 amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further 1 and 90.7% 1 common carp vasa homolog and tilapia vasa homolog for 66.2 1 consensus sequences 1 respectively. In adult tissues 1 specific localization of GgVLG mRNA in the germ cells spermatids and other gonadal somatic cells. Thus 1 the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes less ...

1 ALIMUDDIN, .; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University 1 ANDRIYANI, IRMA; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University 1 ARFAH, HARTON; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University 1 CARMAN, ODANG; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University 1 JUNIOR, MUHAMMAD ZAIRIN; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University 1 OCTAVERA, ANNA; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University more ... 1 YOSHIZAKI, GORO; Department of Marine Biosciences, Tokyo University of Marine Science and Technology less ...

1 eng

1 2011

1 Bogor Agricultural University, Indonesia