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Trehalose preincubation increases mesenchymal (CD271+) stem cells post-cryopreservation viability

Tersimpan di:
Main Authors: Kusuma, Indra; Faculty of Medicine, Universitas YARSI, Jakarta, Hadi, Restu S.; Faculty of Medicine, Universitas YARSI, Jakarta, Kiranadi, Bambang; Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Boediono, Arief; Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor
Other Authors: Lembaga Pengelola Dana Pendidikan
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Faculty of Medicine Universitas Indonesia , 2016
Subjects:
cryopreservation
mesenchymal (CD271+) stem cells
trehalose preincubation
Online Access: http://mji.ui.ac.id/journal/index.php/mji/article/view/1273
http://mji.ui.ac.id/journal/index.php/mji/article/view/1273/1138
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Internet

http://mji.ui.ac.id/journal/index.php/mji/article/view/1273
http://mji.ui.ac.id/journal/index.php/mji/article/view/1273/1138

Lokasi

Koleksi Medical Journal of Indonesia
Gedung Perpustakaan Universitas Indonesia
Institusi Universitas Indonesia
Kota KOTA DEPOK
Provinsi JAWA BARAT
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Lihat Juga

  • Benefit of trehalose preincubation prior to cryopreservation
    oleh: Pawitan, Jeanne A.; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta
    Terbitan: (2016)
  • Peripheral Blood Mesenchymal Stem Cells Isolated from Indonesia Long Tailed Monkey (Macaca fascicularis)
    oleh: Harsoyo, Agus; Primatology Doctoral Program. Bogor Primates Research Center, Research Institutions and Community Service, Bogor Agricultural University, Indonesia, et al.
    Terbitan: (2018)
  • AH-03 Anatomical Characteristic of Forelimb Skeleton of Sumatran Rhino (Dicerorhinus sumatrensis)
    oleh: Nurhidayat, Nurhidayat; Department of Anatomy Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, et al.
    Terbitan: (2017)
  • Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization
    oleh: NURBARIAH, .; 1Department of Anatomy, Physiology and Pharmacology,Faculty of Veterinary Medicine, Bogor Agricultural University, et al.
    Terbitan: (2012)
  • AH-04 Anatomical Characteristic of the Pelvic and Thigh Region Muscles of Javan Pangolin, Manis javanica
    oleh: Nisa', Chairun; Department of Anatomy Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, et al.
    Terbitan: (2017)
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