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The acute toxicity of isolates from n-hexane and ethanolic fraction of Dendrophthoe pentandra (L.) Miq. which have immunostimulatory activity

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Main Authors: ., Katrin; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok Indonesia, Soemardji, Andreanus A.; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Bandung Indonesia, Soeganda, Asep Gana; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Bandung Indonesia, Soediro, Iwang; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Bandung Indonesia
Format: Article info Journal
Bahasa: eng
Terbitan: Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia , 2005
Online Access: http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/431
http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/431/310
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Internet

http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/431
http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/431/310

Lokasi

Koleksi Indonesian Journal of Pharmacy
Gedung Perpustakaan Pusat Universitas Gadjah Mada
Institusi Universitas Gadjah Mada
Kota SLEMAN
Provinsi DAERAH ISTIMEWA YOGYAKARTA
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  • Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers
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    Terbitan: (2013)
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