Lihat Juga

• Three isolates among 138 sponge-associated bacteria were isolated from Waigeo Island, Raja Ampat West Papua Province, Indonesia, have been shown protease inhibitory activity against subtilisin (serine protease), thermolysin (metalloprotease), and crude extract from pathogenic bacteria (Eschericia coli enteropathogenic/EPEC K.1.1, Staphylococcus aureus, and Pseudomonas aeruginosa). Those three isolates were designated as sponge associated bacteria SAB S-12, SAB S-21, and SAB S-17. A simple casein and Sea Water Complete (SWC) double layer agar method was used to screen the bacteria against pathogenic bacteria producing protease, i.e. EPEC K.1.1, S. aureus, and P. aeruginosa. Among them, SAB S-12 isolate showed no inhibitory zone indicated. The isolate had the highest inhibitory activity against subtilisin and crude extract enzyme of pathogenic bacteria, the inhibitory activity was 91.6 and 98.9%, respectively. In addition, the SAB S-21 isolate had the highest inhibitory activity against thermolysin, it was 70.4%. The optimum pH and temperature for protease inhibition of the three isolates was at pH 7.0-8.0 and 40-50 oC respectively. Based on 16S rRNA gene sequence, the closest related with SAB S-12, SAB-17, and SAB-21 isolates was Providencia sp. (92% identity), Paracoccus sp. (86% identity), and Bacillus sp. (% identity), respectively
  oleh: WAHYUDI, ARIS TRI; Department of Biology, Bogor Agricultural University, et al.
  Terbitan: (2011)
• Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island
  oleh: Sipriyadi, Sipriyadi, et al.
  Terbitan: (2022)
• Preliminary Characterization of Protease Inhibitor from Bacteria-Associated with Sponge from Panggang Island, Seribu Islands
  oleh: Nurhayati, Tati, et al.
  Terbitan: (2011)
• Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
  oleh: Yuratmoko, Deri, et al.
  Terbitan: (2011)
• Preliminary Characterization of Protease Inhibitor from Bacteria-Associated with Sponge from Panggang Island, Seribu Islands
  oleh: NURHAYATI, TATI, et al.
  Terbitan: (2009)