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The human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth. In this study, we collected a total of 21 isolates from human feet, and 37 isolates from human mouth. These isolates were identified with biochemical test such as oxidase and catalase test and Gram staining assay. The presence of mxaF gene of methanol dehydrogenase was detected by PCR using specific primers. However, the result showed that most of the isolates did not possess mxaF gene. Hence, the methanol dehydrogenase (MDH) activity was also determined. From the total 21 isolates obtained from the feet, only 15 of them showed MDH activity whereas 23 isolates from the total 37 isolates obtained from teeth and tongue region also showed MDH activity. Isolate K25-3 (74.444 U/ml), K33-6 (79.815 U/ml), and K43-5 (69.259 U/ml) from human feet and M41L3 (135.926 U/ml), M27G2 (85.556 U/ml), and M51G1 (103.333 U/ml) from human mouth showed the highest total enzyme activity. Isolates with the highest total activity could be used for further studies such as purification of the enzyme and isolates characterization

Main Authors: WATURANGI, DIANA ELIZABETH; Atma Jaya Catholic University of Indonesia, NICHOLAS, CATHERINE DELANY; Atma Jaya Catholic University of Indonesia, SUSANTO, CINDY OKTAVIA; Atma Jaya Catholic University of Indonesia, SUHARTONO, MAGGY THENAWIJAYA; Atma Jaya Catholic University of Indonesia
Other Authors: line-height:115%, ">The human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth. In this study, we collected a total of 21 isolates from human feet, and 37 isolates from human mouth. These isolates were identified with biochemical test such as oxidase and catalase test and Gram staining assay. The presence of mxaF gene of methanol dehydrogenase was detected by PCR using specific primers. However, the result showed that most of the isolates did not possess mxaF gene. Hence, the methanol dehydrogenase (MDH) activity was also determined. From the total 21 isolates obtained from the feet, only 15 of them showed MDH activity whereas 23 isolates from the total 37 isolates obtained from teeth and tongue region also showed MDH activity. Isolate K25-3 (74.444 U/ml), K33-6 (79.815 U/ml), and K43-5 (69.259 U/ml) from human feet and M41L3 (135.926 U/ml), M27G2 (85.556 U/ml), and M51G1 (103.333 U/ml) from human mouth showed the highest total enzyme activity. Isolates with the highest total activity could be used for further studies such as purification of the enzyme and isolates characterization.
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Bogor Agricultural University, Indonesia , 2011
Subjects:
The human feet and mouth are known as sources of methylated sulfides
which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria
which may result in odor reduction in human feet and mouth. In this study
we collected a total of 21 isolates from human feet
and 37 isolates from human mouth. These isolates were identified with biochemical test such as oxidase and catalase test and Gram staining assay. The presence of mxaF gene of methanol dehydrogenase was detected by PCR using specific primers. However
the result showed that most of the isolates did not possess mxaF gene. Hence
the methanol dehydrogenase (MDH) activity was also determined. From the total 21 isolates obtained from the feet
only 15 of them showed MDH activity whereas 23 isolates from the total 37 isolates obtained from teeth and tongue region also showed MDH activity. Isolate K25-3 (74.444 U/ml)
K33-6 (79.815 U/ml)
and K43-5 (69.259 U/ml) from human feet and M41L3 (135.926 U/ml)
M27G2 (85.556 U/ml)
and M51G1 (103.333 U/ml) from human mouth showed the highest total enzyme activity. Isolates with the highest total activity could be used for further studies such as purification of the enzyme and isolates characterization
Online Access: http://journal.ipb.ac.id/index.php/hayati/article/view/%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3Bline-height%3A115%25%3B%22%3EThe%20human%20feet%20and%20mouth%20are%20known%20as%20sources%20of%20methylated%20sulfides%2C%20which%20are%20produced%20by%20other%20microflora.%20Methylated%20sulfides%20could%20be%20oxidized%20by%20methylotrophic%20bacteria%2C%20which%20may%20result%20in%20odor%20reduction%20in%20human%20feet%20and%20mouth.%20In%20this%20study%2C%20we%20collected%20a%20total%20of%2021%20isolates%20from%20human%20feet%2C%20and%2037%20isolates%20from%20human%20mouth.%20These%20isolates%20were%20identified%20with%20biochemical%20test%20such%20as%20oxidase%20and%20catalase%20test%20and%20Gram%20staining%20assay.%20The%20presence%20of%20%3Cem%3EmxaF%3C%2Fem%3E%20gene%20of%20methanol%20dehydrogenase%20was%20detected%20by%20PCR%20using%20specific%20primers.%20However%2C%20the%20result%20showed%20that%20most%20of%20the%20isolates%20did%20not%20possess%20%3Cem%3EmxaF%3C%2Fem%3E%20gene.%20Hence%2C%20the%20methanol%20dehydrogenase%20%28MDH%29%20activity%20was%20also%20determined.%20From%20the%20total%2021%20isolates%20obtained%20from%20the%20feet%2C%20only%2015%20of%20them%20showed%20MDH%20activity%20whereas%2023%20isolates%20from%20the%20total%2037%20isolates%20obtained%20from%20teeth%20and%20tongue%20region%20also%20showed%20MDH%20activity.%20Isolate%20K25-3%20%2874.444%20U%2Fml%29%2C%20K33-6%20%2879.815%20U%2Fml%29%2C%20and%20K43-5%20%2869.259%20U%2Fml%29%20from%20human%20feet%20and%20M41L3%20%28135.926%20U%2Fml%29%2C%20M27G2%20%2885.556%20U%2Fml%29%2C%20and%20M51G1%20%28103.333%20U%2Fml%29%20from%20human%20mouth%20showed%20the%20highest%20total%20enzyme%20activity.%20Isolates%20with%20the%20highest%20total%20activity%20could%20be%20used%20for%20further%20studies%20such%20as%20purification%20of%20the%20enzyme%20and%20isolates%20characterization.%3C%2Fspan%3E%3C%2Fstrong%3E
http://journal.ipb.ac.id/index.php/hayati/article/view/%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3Bline-height%3A115%25%3B%22%3EThe%20human%20feet%20and%20mouth%20are%20known%20as%20sources%20of%20methylated%20sulfides%2C%20which%20are%20produced%20by%20other%20microflora.%20Methylated%20sulfides%20could%20be%20oxidized%20by%20methylotrophic%20bacteria%2C%20which%20may%20result%20in%20odor%20reduction%20in%20human%20feet%20and%20mouth.%20In%20this%20study%2C%20we%20collected%20a%20total%20of%2021%20isolates%20from%20human%20feet%2C%20and%2037%20isolates%20from%20human%20mouth.%20These%20isolates%20were%20identified%20with%20biochemical%20test%20such%20as%20oxidase%20and%20catalase%20test%20and%20Gram%20staining%20assay.%20The%20presence%20of%20%3Cem%3EmxaF%3C%2Fem%3E%20gene%20of%20methanol%20dehydrogenase%20was%20detected%20by%20PCR%20using%20specific%20primers.%20However%2C%20the%20result%20showed%20that%20most%20of%20the%20isolates%20did%20not%20possess%20%3Cem%3EmxaF%3C%2Fem%3E%20gene.%20Hence%2C%20the%20methanol%20dehydrogenase%20%28MDH%29%20activity%20was%20also%20determined.%20From%20the%20total%2021%20isolates%20obtained%20from%20the%20feet%2C%20only%2015%20of%20them%20showed%20MDH%20activity%20whereas%2023%20isolates%20from%20the%20total%2037%20isolates%20obtained%20from%20teeth%20and%20tongue%20region%20also%20showed%20MDH%20activity.%20Isolate%20K25-3%20%2874.444%20U%2Fml%29%2C%20K33-6%20%2879.815%20U%2Fml%29%2C%20and%20K43-5%20%2869.259%20U%2Fml%29%20from%20human%20feet%20and%20M41L3%20%28135.926%20U%2Fml%29%2C%20M27G2%20%2885.556%20U%2Fml%29%2C%20and%20M51G1%20%28103.333%20U%2Fml%29%20from%20human%20mouth%20showed%20the%20highest%20total%20enzyme%20activity.%20Isolates%20with%20the%20highest%20total%20activity%20could%20be%20used%20for%20further%20studies%20such%20as%20purification%20of%20the%20enzyme%20and%20isolates%20characterization.%3C%2Fspan%3E%3C%2Fstrong%3E/2126

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http://journal.ipb.ac.id/index.php/hayati/article/view/%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3Bline-height%3A115%25%3B%22%3EThe%20human%20feet%20and%20mouth%20are%20known%20as%20sources%20of%20methylated%20sulfides%2C%20which%20are%20produced%20by%20other%20microflora.%20Methylated%20sulfides%20could%20be%20oxidized%20by%20methylotrophic%20bacteria%2C%20which%20may%20result%20in%20odor%20reduction%20in%20human%20feet%20and%20mouth.%20In%20this%20study%2C%20we%20collected%20a%20total%20of%2021%20isolates%20from%20human%20feet%2C%20and%2037%20isolates%20from%20human%20mouth.%20These%20isolates%20were%20identified%20with%20biochemical%20test%20such%20as%20oxidase%20and%20catalase%20test%20and%20Gram%20staining%20assay.%20The%20presence%20of%20%3Cem%3EmxaF%3C%2Fem%3E%20gene%20of%20methanol%20dehydrogenase%20was%20detected%20by%20PCR%20using%20specific%20primers.%20However%2C%20the%20result%20showed%20that%20most%20of%20the%20isolates%20did%20not%20possess%20%3Cem%3EmxaF%3C%2Fem%3E%20gene.%20Hence%2C%20the%20methanol%20dehydrogenase%20%28MDH%29%20activity%20was%20also%20determined.%20From%20the%20total%2021%20isolates%20obtained%20from%20the%20feet%2C%20only%2015%20of%20them%20showed%20MDH%20activity%20whereas%2023%20isolates%20from%20the%20total%2037%20isolates%20obtained%20from%20teeth%20and%20tongue%20region%20also%20showed%20MDH%20activity.%20Isolate%20K25-3%20%2874.444%20U%2Fml%29%2C%20K33-6%20%2879.815%20U%2Fml%29%2C%20and%20K43-5%20%2869.259%20U%2Fml%29%20from%20human%20feet%20and%20M41L3%20%28135.926%20U%2Fml%29%2C%20M27G2%20%2885.556%20U%2Fml%29%2C%20and%20M51G1%20%28103.333%20U%2Fml%29%20from%20human%20mouth%20showed%20the%20highest%20total%20enzyme%20activity.%20Isolates%20with%20the%20highest%20total%20activity%20could%20be%20used%20for%20further%20studies%20such%20as%20purification%20of%20the%20enzyme%20and%20isolates%20characterization.%3C%2Fspan%3E%3C%2Fstrong%3E/2126

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