Lihat Juga

• In vitro regeneration of sugarcane (Saccharum officinarumL.) clones can support breeding program of sugarcane. This research was conducted to study the effect of benzyladenine on in vitro shoot formation from sugarcane callus. Leafrolls were cultured for 8 weeks on callus induction medium containing MS salts, 30 g L-1 sucrose, 150 ml L-1 coconut water, 100 mg L-1 myo-inositol, 0.1 mg L-1 thiamine-HCl, 0.5 mg L-1 pyridoxine-HCl, 0.5 mg L-1 nicotinic acid, 2 mg L-1 glycine, and 3 mg L-1 2,4-D. Callus was then subjected to different concentrations of benzyladenine (BA) (0, 0.5, 1, 2, and 2.5 mg L-1) contained in MS media. The experiment showed that after 8 weeks in culture 2 and 2.5 mg L-1 BA led to the highest percentage of shoot formation (100%). The experiment also showed that addition of BA caused an increase in percentage of shoot formation, number of shoot per callus clumps, and average shoot length. In the range of 0-2.5 mg L-1, the higher the concentrations of BA the more shoots and the longer shoots were produced. Highest number of shoots was recorded at BA 2.5 mg L-1 (36.4 shoots per callus clump) and highest average shoot length was obtained at 2 and 2.5 mg L-1 BA, i.e. 2.25 and 2.3 cm respectively. The shoot formation system was then applied to 12 sugarcane genotypes, resulting in statistically different response and producing substantial number of shoots, ranging from 29 to 41.33 shoots per clump. Keywords: In vitro, sugarcane, leafrolls, benzyladenine, shoot formation
  oleh: Hapsoro, Dwi; Department of Agronomy, Faculty of Agriculture, University of Lampung
• Induction Shoots From Callus of Cucumber Apple (Cucumis sp.) Using a Combination of Benzil Amino Purine and Naphtalene Acetic Acid Concentrations In Vitro
  oleh: Natalia Erzalinda Mendrofa, Fransisca, et al.
  Terbitan: (2021)
• Effect of Genotype, Explant Type and Growth Regulators on The Accumulation of Flavonoides of (Silybum marianum L.) in In vitro Culture
  oleh: A. Pourjabar, et al.
  Terbitan: (2012)
• IIn Vitro Selection of Four Soybean Genotypes using PEG for Drought Tolerance
  oleh: Saepudin, Adam; Departement of Agrotechnology, Faculty of Agriculture, Siliwangi University, et al.
  Terbitan: (2017)
• This research was to study in vitro callus induction and somatic embryogenesis in oil palm from leaf explants. Young leaf segments from mature oil palm were cultured on MS medium supplemented with different concentrations of 2,4-D with or without addition of 2 g/l activated charcoal (AC) or 2,4-D and picloram. Embryogenesis induction was done using MS medium containing 2,4-D 450 mM and benziladenine 4.4 mM with 3g/l activated charcoal. The treatment of 2,4-D 15 mM resulted in the highest percentage of callus induction. The treatment of 2,4-D and AC showed that 2,4-D 450 mM and AC led to higher percentage of callus induction than that of 2,4-D 400 mM and 2 g/l AC. Embryogenesis occured in 27 out of 250 clumps of primary callus was occurred after 2-3 times subcultures. Somatic embryo development occurred when the embryogenic callus was transferred on the same basal medium supplemented with casein hydrolysate with 1 mM BA or growth regulator free basal medium with 2 g/l activated charcoal
  oleh: YUSNITA, .; The University of Lampung, et al.
  Terbitan: (2011)