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Construction of RNA Interference Vector to Silence Aluminum Tolerance Gene Candidate in Rice cv Hawara Bunar

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Main Authors: Wahyuningtyas, Windarti; Plant Biology Graduate Program, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Miftahudin, Miftahudin; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Widyastuti, Utut; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Tjahjoleksono, Aris; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Bogor Agricultural University, Indonesia , 2016
Subjects:
3′UTR_B11
Al tolerant
rice
RNAi vector
Online Access: http://journal.ipb.ac.id/index.php/hayati/article/view/13940
http://journal.ipb.ac.id/index.php/hayati/article/view/13940/10451
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Internet

http://journal.ipb.ac.id/index.php/hayati/article/view/13940
http://journal.ipb.ac.id/index.php/hayati/article/view/13940/10451

Lokasi

Koleksi Jurnal Matematika dan Aplikasinya
Gedung Perpustakaan Institut Pertanian Bogor
Institusi Institut Pertanian Bogor
Kota BOGOR
Provinsi JAWA BARAT
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Lihat Juga

  • Overexpression of B11 Gene in Transgenic Rice Increased Tolerance to Aluminum Stress
    oleh: Siska, Devi Media; Graduate Program in Plant Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Darmaga, Bogor 16680, et al.
    Terbitan: (2018)
  • GENE IDENTIFICATION AT THE INSERTION SITE OF A HIGH SALINITY TOLERANT INSERTIONAL MUTANT RICE LINE
    oleh: Zannati, Anky; Pusat Penelitian Bioteknologi LIPI cibinong, et al.
    Terbitan: (2018)
  • Physiological and Biochemical Responses to Aluminum Stress in the Root of a Biodiesel Plant Jatropha curcas L.
    oleh: TISTAMA, RADITE; Department of Biology, Faculty of Mathemathics and Natural Sciences, Bogor Agriculture University. Research Center for Bioresources and Biotechnology, Bogor Agricultural University, et al.
    Terbitan: (2012)
  • Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers
    oleh: HASTUTI, RATIH DEWI; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, et al.
    Terbitan: (2013)
  • Metagenomic of Actinomycetes Based on 16S rRNA and nifH Genes in Soil and Roots of Four Indonesian Rice Cultivars Using PCR-DGGE
    oleh: Mahyarudin, .; Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University, IPB Dramaga Campus, Bogor, West Java 16680, et al.
    Terbitan: (2015)
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