Additive Main Effects Multiplicative Interaction (AMMI) is a widely known analysis used in understanding genotype and environment interaction (GEI) in plant breeding research. The interpretation of AMMI based on biplot visualizes the first two component of principle components analysis. Biplot of AMMI is only an exploration analysis and it does not provide the hypothesis testing, so it can conduct different interpretation by plant breeding researchers. The aim of this research is to find a systematic approach through bootstrap resampling method. Bootstrap resampling method in AMMI model produces confidence region of the first two interaction principle component ( and ) for genotype and environment respectively. Bootstrap confidence region of and estimated the stability of genotype, thus making AMMI analysis more precise and realiable for characterization and selection of genetic environment